CRISPR Design

Compact design workspace for Brassica napus sgRNA tasks. Configure inputs, run async scoring, and inspect candidates with transparent off-target evidence.

Submit Task

1. Target Input + Core Design Settings gene_id

Input Type

Genome

Gene ID

Legacy Genome Retriever

Input symbol/ATG ID, then search to resolve legacy genome Gene IDs.

No candidates loaded.

Symbol / ATG ID Resolver

Resolve symbols and/or paste custom genes below.

No homoeolog candidates loaded.

Target Gene IDs

Chr

Start

End

Chr Position mode submits as chr:start-end to API.

FASTA Sequence

Cas Type

PAM

On-target Model

Spacer Length

2. Advanced Constraints and Oligo Options

Target Region

Promoter Constraint

Cas-OFFinder Guide Limit

Tolerant mode (show guides even with incomplete/off-target-heavy profile)

Oligo Template

Include synthetic oligos output

Strict promoter-template compatibility

Restriction Presets

Custom Motifs

Window Upstream

Window Downstream

Result

Mode: CDS only guides=0 offtargets=0

Gene Target View

High-quality guide Standard guide 5' UTR CDS 3' UTR Intron

Run a task and load candidates to render the gene target track.

All-copy Insights

Run an all-copy task to inspect target coverage summary.

Coverage Matrix

Coverage matrix will appear after all-copy candidates are loaded.

On-target sgRNA List

Sequence (|=cut)

Final

On-target

Region

%GC

No guides loaded.

Guide Detail View

Click a guide sequence from the left to inspect details.

Off-target Detail

Guide-specific off-target rows will appear here.